Expert Solutions for Oligonucleotide Discovery, Development, and Analysis

Comprehensive Support for Oligonucleotide Development

From preclinical to clinical, our laboratory scientific experts support robust and sensitive bioanalytical assays that minimize interference for accurate oligonucleotide bioanalysis and quantification.

Beyond the core analytical techniques, Altasciences provides various services to support the oligonucleotide development journey.

• Immunogenicity Testing: We offer immunogenicity/anti-drug antibody assay development and validation to assess the potential immune response to oligonucleotide therapies.
• PCR Analysis: Our expertise extends to various PCR techniques, including real-time PCR and dPCR, for sensitive and specific detection of oligonucleotide sequences.
• Tissue-Based Analysis: We have the capabilities to perform tissue-based analysis, enabling the quantification and characterization of oligonucleotides in target tissues.
• Regulatory Guidance: Our team provides expert guidance on navigating the complex regulatory landscape for oligonucleotides and other cell and gene therapies, ensuring compliance and facilitating successful development programs.

bioanalytical techniques and platforms for oligonucleotides:

• Meso Scale Discovery (MSD™) multiplexing technology utilizing the electroluminescence-based detection system 
• Mass spectrometry-based quantification of linear and conjugated oligos (ASO, siRNA, aptamer) using solid phase extraction (SPE) or automated hybridization LC-MS/MS workflow
• In vitro plasma stability, metabolite identification, and plasma protein binding studies using mass spectrometry platforms
• Technology selection advice based upon sensitivity, selectivity, complexity, dynamic range, throughput and specific program needs